‘DNA bisulfite pyrosequencing – from whole genome to targeted gene methylation analysis’ workshop takes place at the Institute of Human Genetics PAS in time period 6 – 9 June 2022. During a 5-day wet lab workshop, participants will have the opportunity to learn this technique step by step, starting with designing assays, data analysis and interpretation of results. We will particularly focus on three pyrosequencing applications (I) genotyping, (II) DNA methylation analysis and (III) cfDNA methylation analysis / working with low DNA concentrations.
Abstract:
Recurrent epigenetic alterations in hematological malignancies like DNA methylation changes can be used as powerful biomarkers. A suitable technic that allows global as well as targeted quantification of DNA methylation levels in cell lines, tumor samples, microdissected cells but also cfDNA is bisulfite pyrosequencing. During the planned workshop we will share our long-term experience in pyrosequencing both on its straightforward application for genotyping but also more demanding applications like cfDNA methylation analysis.